Ap bio pglo transformation formal lab report

Then the data wont be reliable. Coli is unable to sustain itself and dies off.

Ap bio pglo transformation formal lab report

When the 50 seconds were done, both tubes were placed back on ice with a rapid change. Also, if the incubation time wasnt exact, thats also room for error. However, the modified E. Lesson 3 Review Questions Do you observe some E. I can speculate that the answer behind this question is the heat shock, which was a very crucial part of the experiment as it was the specific point where the pGLO plasmid DNA would enter the E. Bacteria colonies are clustered. There is a lot of growth. One very particular thing I noticed when observing the three ager plates with the live bacteria was that the bacteria without pGLO was greasier and messier then the bacteria with the pGLO plasmid DNA. Based on the results from this experiment, there is an incredible advantage to turning on and off genes.

One very particular thing I noticed when observing the three ager plates with the live bacteria was that the bacteria without pGLO was greasier and messier then the bacteria with the pGLO plasmid DNA.

More scattered 2. Using 4 new and unused loops for each ager plate, we spread around the bacteria in the plate. B What do you think each of the two environmental factors listed above are doing to cause the genetically transformed bacteria turn green?

So to explain the relatively small transformation efficiencies of some the groups above, I believe it came from not stirring up the solution well enough before the heat shock and since the experiment was working with tiny amounts of various substances, every little bit matters.

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From the results of the experiment, it is clearly possible to change bacteria and thus manipulate its physical capabilities. In the pGLO lab, scientist were able to clone the GFP gene from a jellyfish known as Aequorea victoria, and from this they were able to make a recombinant plasmid, which is called pGLO. Lastly, the modified E. An organism which reproduces quickly, like bacteria. From your results, can you tell if these bacteria are ampicillin resistant by looking at them on the LB plate? The tube was placed back in the tube rack in the ice. In our results, we found that the only condition in which the E. Using 4 new and unused loops for each ager plate, we spread around the bacteria in the plate. The control plates are the pGLO plates, so that the plates can be compared to see if the bacteria were really transformants. Additionally, if the E. But despite this fact, the experiment overall was a success as all groups were able to get at least some transformed bacteria. Yes, the bacteria without plasmids in the plain LB plate lawn. The UV light helps fluoresce the protein. As expected, there is no growth at all.

Coli bacteria into the micro test tubes. Scientists often want to know if the genetically transformed organism can pass its new traits on to its offspring and future generations.

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Green Fluorescent Protein drawn in cartoon style w Because the environment contained the arabinose sugar, an ARA inducer molecule was able to inactivate the repressor and allow transcription of the inserted GTP molecule to commence.

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AP Lab #6: pGLO Transformation Lab